Advances in Agronomy and Plant Science
Volume 1 | Issue 1 | Pages 01-12
Genotypic variation to aluminium sensitivity in chickpea depends on its ability to efficiently accumulate nitrate
Manorma Sharma 1, Marta Trofimova 2, Vinay Sharma 1 and Bhumi Nath Tripathi 1, 3, 4*
1 Department of Bioscience and Biotechnology, Banasthali University, Banasthali, 304022, Rajasthan, India.
2 Department of Environmental Studies, National University of Kyiv-Mohyla Academy, 2 Skovorody vul., 04655, Kyiv, Ukraine.
3 Department of Botany, Guru Ghasidas University, Bilaspur, 495009, Chhattisgarh, India.
4 Academy of Innovative Research, Bemawal (Ambedkarnagar), 224181, Uttar Pradesh, India.
Aluminium toxicity is the major agricultural problem severely limiting the crop production in acidic soil. Chickpea is one of the most important legumes and widely cultivated in acid prone soil. But our knowledge about the interaction of chickpea with aluminium is very limited. The present study is the first report demonstrating the genotypic variation in Cicer arietinum (chickpea) against Al stress. This study also proposes that chickpea genotypes with efficient nitrate accumulation ability are more capable to tolerate Al stress. For this purpose, total 24 genotypes of chickpea were assessed for their sensitivity against Al. Based on Al-mediated inhibition of root elongation, three least inhibited (RSG 974, RSG 963 and RSG 896) and three most inhibited (RSG 945, CSJ 573 and CSJ 513) chickpea genotypes were selected as Al-tolerant and Al-sensitive genotypes, respectively, for further analysis. The intensity of hematoxylin staining (indirect indicator of Al accumulation in the root tissue) was lower in Al-tolerant than Al-sensitive genotypes, indicating lesser accumulation of Al by Al-tolerant genotypes. However, accumulation of phosphate did not show any differential pattern between Al-tolerant and sensitive genotypes. But all Al-tolerant genotypes showed increased uptake of nitrate during Al-stress, whereas nitrate uptake was mostly decreased or remained unchanged in Al-sensitive genotypes of chickpea. Based on available data the present study speculated that efficient accumulation of nitrate somehow improves the Al tolerance in Cicer arietinum (chickpea).